Composite

Part:BBa_K3924032:Design

Designed by: Yu Meng   Group: iGEM21_Tsinghua   (2021-10-10)


Pro-LAC, specifically labelling RNA by sgRNA guiding under blue light illumination


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
    Illegal PstI site found at 682
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
    Illegal NheI site found at 4
    Illegal PstI site found at 682
    Illegal NotI site found at 1102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
    Illegal BamHI site found at 37
    Illegal XhoI site found at 1086
    Illegal XhoI site found at 1111
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
    Illegal PstI site found at 682
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
    Illegal PstI site found at 682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 950


Design Notes

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Source

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References